Short Communication

Standardization/optimization of High Quality DNA Isolation Protocal by using CTAB Method

THOMBARE DEVIDAS, PERWEEN SABIHA, PRASAD ARCHANA & VERULKAR SATISH

Department of Plant Molecular Biology and Biotechnology, College of Agriculture Indira Gandhi Krishi Vishwavidylaya, Raipur, Chhattisgarh

Corresponding Author's Email: devidas202013@gmail.com

Abstract

The rice is important food in world population need to improved crops and food productivity. DNA based breeding selection is much more reliable other than traditional breeding methods. Howere, mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including ├╗avonoids and other secondary metabolites which interfere with the extraction of genomic DNA. The extraction of DNA is often the most time consuming and laborious step in highthrough put molecular genetic analysis. This study a rapid and reliable cetyl trimethylammonium bromide (CTAB) protocol suited specifically for extracting genomic DNA from rice plants. The purity of extracted DNA was excellent as evident by A260/A280 ratio ranging from 1.78 to 1.84 and A260/A230 ratio was good quality and yield assessments, electrophoresis was done of all DNA samples in 0.8% agarose gel, stained with Ethidium Bromide and bands were observed in gel documentation system.

Keywords: DNA extraction, high-throughput PCR, marker assisted selection, gene mapping

Page No.: 3538-3540

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